Non-hazardous pest control

ABSTRACT

A pesticide and a method of using the pesticide to kill invertebrates, especially insects, arachnids and larvae. The method is to prepare a mixture of a carrier with the pesticide and apply the mixture to the insects, arachnids and larvae and their habitat. The pesticide is a neurotransmitter affector utilizing octopamine receptor sites in the insects, arachnids and larvae. The affector agent is a chemical having a six membered carbon ring having substituted thereon at least one oxygenated functional group. The affector agent is a chemical component of a plant essential oil and is naturally occurring. Deposition of the chemicals of the present invention on a surface provides residual toxicity for up to 30 days. Various carriers for the affector agent are disclosed. The chemicals of the present invention deter feeding of insects, arachnids and larvae and also retard growth of the larvae of the insects and arachnids.

CROSS REFERENCE TO RELATED APPLICATIONS

The present invention is a continuation-in-part of application Ser. No. 08/657,585, filed Jun. 6, 1996, which is a continuation-in-part of Ser. No. PCT/US94/05823, filed May 20, 1994, now in the U.S. national phase Ser. No. 08/553,475, filed Nov. 9, 1995; which is, in turn, a continuation-in-part of U.S. patent application Ser. No. 08/065,594, filed May 21, 1993, which has subsequently issued as U.S. Pat. No. 5,439,690, issued Aug. 8, 1995.

BACKGROUND OF THE INVENTION

The present invention relates to a method of controlling pests and more particularly to a method of preparing and applying a pesticide which affects octopamine receptor sites in insects, arachnids and larvae.

Many chemicals and mixtures have been studied for pesticidal activity for many years with a goal of obtaining a product which is selective for invertebrates such as insects, arachnids and larvae thereof and has little or no toxicity to vertebrates such as mammals, fish, fowl and other species and does not otherwise persist in and damage the environment. Most products of which the applicants are aware and which have sufficient pesticidal activity to be of commercial significance, also have toxic or deleterious effects on mammals, fish, fowl or other species which are not the target of the product. For example, organophosphorus compounds and carbamates inhibit the activity of acetylcholinesterase in insects as well as in all classes of animals. Chlordimeform and related formamidines are known to act on octopamine receptors of insects but have been removed from the market because of cardiotoxic potential in vertebrates and carcinogenicity in animals and a varied effect on different insects. Also, very high doses are required to be toxic for certain insect species.

It is postulated that amidine compounds affect the octopamine sensitive adenylate cyclase present in insects [Nathanson et al, Mol. Parmacol 20: 68-75 (1981) and Nathanson, Mol. Parmacol 28: 254-268 (1985)]. Another study was conducted on octopamine uptake and metabolism in the insect nervous system [Wierenga et al, J Neurochem 54, 479-489 (1990)]. These studies were directed at nitrogen containing compounds which mimic the octopamine structure.

Insecticides such as trioxabicyclooctanes, dithianes, silatranes, lindane, toxaphen, cyclodienes and picrotoxin act on the GABA (gamma amino butyric acid) receptor. However, these products also affect mammals, birds, fish and other species.

There is a need for a pesticide which targets only insects, arachnids and their larvae and does not produce unwanted and harmful affects on other species.

BRIEF SUMMARY OF THE INVENTION

It is a primary object of the present invention to provide a method of preparing and applying a pesticide which kills invertebrates, especially insects, arachnids and their larvae and has no harmful effects on other species including mammals, fish and fowl.

It is a further object of the present invention to provide a method of preparing and applying a pesticide which exerts its pesticidal properties through the octopamine receptor site in insects, arachnids and their larvae and other invertebrates.

It is still another object of the present invention to provide a method of preparing and applying a pesticide at relatively low concentrations which will be effective over a comparatively long period of time such as at least 24 hours.

In accordance with the teachings of the present invention, there is disclosed a method of killing insects and arachnids and larvae thereof. The steps include preparing a mixture of a carrier with an affector agent which interferes with the neurotransmitters of the octopamine receptor site in insects, arachnids and their larvae and applying the mixture to insects, arachnids, larvae and their habitat. The affector agent interacts with octopamine receptor sites in the insects, arachnids and larvae and interferes with neurotransmission in the invertebrate but does not affect mammals, fish and fowl. The agent is a chemical having the structure of a six member carbon ring, the carbon ring having substituted thereon at least one oxygenated functional group.

There is further disclosed a method of killing insects and arachnids and larvae thereof. A blend of cinnamic alcohol, eugenol and alpha terpineol is prepared. The blend is mixed with a carrier to produce a uniform mixture. The mixture is applied to insects and arachnids and larvae and their habitat. The blend interacts with octopamine receptor sites in the invertebrate and interferes with neurotransmission in the invertebrate but does not affect mammals, fish and fowl.

In another aspect, there is disclosed a pesticide which has an affector agent having a six member carbon ring. The carbon ring has substituted thereon at least one oxygenated functional group. The affector agent affects the octopamine receptor site in invertebrates including insects, arachnids and their larvae. The affector agent is intimately mixed with a carrier. Exposure of insects, arachnids and their larvae to the affector agent produces a disruption of the octopamine receptor site in the invertebrates to interfere with neurotransmission in the invertebrate and the death of the exposed invertebrate.

Further disclosed is a method of killing insects, arachnids, and larvae. A mixture is prepared of a chemical derived from a plant essential oil with a carrier. The chemical has at least one oxygenated functional group therein. The chemical has octopamine receptor site inhibitory activity. The mixture is applied to insects, arachnids, larvae and their habitat. The chemical interacts with an octopamine receptor site in the insects, arachnids and larvae and interferes with neurotransmission in the insects, arachnids and larvae thereof but does not affect mammals, fish and fowl.

In another aspect there is disclosed a method of controlling insects, arachnids and their larvae. An emulsion is prepared of an affector agent which disrupts neurotransmission at the octopamine receptor site in insects, arachnids and their larvae. The mixture is applied to insects, arachnids, larvae and their habitat. The agent interacts with octopamine receptor sites in the insects, arachnids and larvae and deters the feeding of the insects, arachnids and larvae but does not affect mammals, fish and fowl.

In yet another aspect there is disclosed a method of controlling insects, arachnids and larvae. An affector agent mixed with a carrier is applied to larvae of the insects and arachnids and their habitat. The affector agent retards the growth of the larvae. The affector agent interacts with octopamine receptor sites in the larvae of the insects and arachnids and interferes with neurotransmission in the larvae but does not affect mammals, fish and fowl. The affector agent is a naturally occurring organic chemical having at least six (6) carbon atoms.

In addition, there is disclosed a method of killing insects, arachnids and larvae thereof. A mixture is prepared of a carrier and a naturally occurring organic chemical having at least six carbon atoms. The chemical has octopamine receptor site inhibitory activity. The mixture is applied to insects and arachnids and larvae thereof and their habitat. The chemical interacts with an octopamine receptor site in the insects and arachnids and larvae thereof and interferes with neurotransmission in the insects, arachnids and larvae thereof but does not affect mammals, fish and fowl.

These and other objects of the present invention will become apparent from a reading of the following specification, taken in conjunction with the enclosed drawings.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 is a graph showing the effect of the 3-B mixture of the present invention on the intracellular concentration of [Ca²⁺ ] in neuronal cells.

FIG. 2 is a chart showing the contraction of cockroach leg muscles induced by electrical stimulation when the 3-B mixture of the present invention is injected into the thorax of a live cockroach.

FIG. 3 is a chart showing the contraction of cockroach leg muscles in an isolated leg induced by electrical stimulation when the 3-B mixture of the present invention is applied to the isolated leg.

FIG. 4 is a chart showing the test of FIG. 2 using acetone as a control without the 3-B mixture.

FIG. 5 is a chart showing the test of FIG. 3 using acetone as a control without the 3-B mixture.

FIG. 6 is a series of charts showing transmission of signals from the mechanoreceptors of the cerci after application of the 3-B mixture and a control to the abdominal nerve of the cockroach.

FIG. 7 is a chart showing feeding damage to cabbage plant leaf discs with the 3-B mixture as compared to a control.

DESCRIPTION

Physiological activity to invertebrate insects, arachnids and their larvae are produced by the chemicals of the present invention and by mixtures of these chemicals. The following chemicals having a six member carbon ring and having substituted thereon at least one oxygenated functional group are representative of the chemicals of the present invention but these are not to be considered as being the totality of chemicals and are not a limitation. ##STR1##

The above-listed materials are all components of plant essential oils. A further plant essential oil which is a chemical of the present invention is ##STR2##

Anethole, carvacrol, citronellal, eugenol, D-pulegone, alpha-terpineol and thymol are all monoterpenes each having ten (10) carbon atoms therein.

The present invention is not limited to the chemicals listed herein.

All of the chemicals of the present invention are naturally occurring organic chemicals which are devoid of halogens. Further, several of the chemicals of the present invention are considered by the U.S. Environmental Protection Agency (EPA) to be safe for humans and exempted from registration. Thus, these chemicals do not require prior approval or registration with the EPA. Some of these chemicals have been added to prior art insecticides as attractants and repellents. However, there have been no reports of these chemicals having toxic pesticidal activity at the concentrations disclosed in the present invention.

All of the chemicals of the present invention act as agonists or antagonists on the octopamine receptor site in insects, arachnids and their larvae and, consequently, produce physiological effects in exposed invertebrates. The chemicals of the present invention are considered to be affector agents. Exposure to reduced concentrations of the chemicals or exposure for brief times affect the feeding habits of the exposed insects, arachnids and larvae. This is important in those invertebrates which feed on vegetation, since there is reduced damage to plants by invertebrates which have received sublethal concentrations of the chemicals of the present invention. Also, the insects, arachnids or larvae which survive exposure to the chemicals of the present invention are shown to be stunted in growth.

Alpha-terpineol, eugenol and cinnamic alcohol were dissolved in acetone and designated as sample 3B. The range of weight percentages of the components of the blend are alpha-terpineol 10%-50%, eugenol 10%-50% and cinnamic alcohol 20%-35%. The preferred blend has equal parts by weight of each of the components. Male and female American cockroaches were injected with 3B into the abdomen. Overt signs of toxicity were observed at 1 mg/roach in 2 μl of acetone. At lower doses no symptoms were observed. By this approach, 2 out of 6 died within 30 minutes. For those surviving, some showed locomotive difficulties. No hyperactivity was observed, even in those that died quickly. In some cases, (2/6) treated insects died after 2 to 3 days.

None of the American cockroaches died when they were treated through topical application prior to 24 hours. A 40% (4/10) mortality was observed 24 hours after treatment and this effect was time dependent. After 72 hours, 100% died. Control insects receiving 2 μl of only acetone through injection showed no ill effects (Table 1).

                  TABLE 1                                                          ______________________________________                                         Time course effect of 3-B (1                                                   mg/insect) on American cockroaches                                             treated by topical application.                                                Time (hr.)   Mortality-3B                                                                             Mortality-Control                                       ______________________________________                                         24           4/10      0                                                       48           6/10      0                                                       72           10/10     0                                                       ______________________________________                                    

The data from the above study suggests that the action of 3B depends on the site of application, i.e., abdominal injection vs. topical application for the whole body.

3B was applied to different areas of the insect. When given to the ventral sternum region (at the base of the hind legs) of the American cockroach, 3B was most toxic at 125 μg/insect. In that case, overt toxic signs were observed within 10 minutes. A lethal dose of 250-500 μg/insect was injected and the insects died within 30 minutes (Table 2).

                  TABLE 2                                                          ______________________________________                                         Time-course and dose-response of 3B                                            on American cockroaches treated by                                             injection through the ventral stenum.                                                 Mortality*                                                              Time   Test Doses μg/insect                                                 (hrs.) 50        100     125      250  500                                     ______________________________________                                         0.5    0         2/10    6/10     10/10                                                                               10/10                                   1.0    2/10      3/10    7/10     --   --                                      1.5    5/10      7/10    9/10     --   --                                      2.0    5/10      9/10    10/10    --   --                                      3.0    6/10      9/10    --       --   --                                      5.0    8/10      10/10   --       --   --                                      24.0   10/10     --      --       --   --                                      ______________________________________                                          *No mortality was found in insects treated exactly in the same manner wit      equal volume of acetone (vehicle) alone as control.                      

In the latter case of injection of a lethal dose the hind legs appeared to be paralyzed and the fore and mid legs moved rapidly, although there was no overt hyperexcitation. This phenomenon of quick death was also observed in German cockroaches that were treated through topical application (3B is more toxic to German than to American cockroaches). At 125 μg/insect, 80% (8/10) of German cockroaches were knocked down in 2 to 3 hours and died within 24 hours (dose was 125 μg 3B per insect with 0.4 μl acetone).

The above data support the observation that site of application gives varying degrees of toxicity. For further confirmation, 1 mg 3B in 20 ml acetone was applied to small jars and all surfaces were covered. One hour after the acetone had completely evaporated, 5 American cockroaches were introduced to each jar. Control jars were treated exactly as above but with 20 ml acetone only. All cockroaches died within 10 to 30 minutes in the jars containing 3B. None died in the control jars. Some insects (3/5) showed hyperactivity within 1 to 3 minutes after exposure. At 8 minutes, hind leg paralysis was observed. These "walk across" data are consistent with the previous study in which a rapid death was observed when 3B was given to the ventral sternum region rather than the abdomen. The fact that the toxicants might penetrate faster through the legs (no chitin layer as in the body) support the notion that the permeability/penetration of 3B plays a key role in its toxicity (Table 3).

                  TABLE 3                                                          ______________________________________                                         Time-course effect of 3B (1 mg/jar)                                            on American cockroaches exposed to                                             pre-treated surface one hour after 3-                                          B application.*                                                                       Time (min.)                                                                            Mortality                                                       ______________________________________                                                10      3/5                                                                    15      3/5                                                                    20      4/5                                                                    30      5/5                                                             ______________________________________                                    

Based on these data, the same treated jars were used for a residual study. In this experiment, American cockroaches were transferred to the treated and control jars at different times from the point in time at which acetone evaporated, i.e., 24, 48, 96, 72 hours and 7 days. Interestingly, all insects died after being exposed to treated jars, even 7 days after 3B application. However, it took longer for the roaches to die in proportion to the length of time from initial 3B application to the jar. Some toxic signs were observed in all cases after exposure. Further, when the same experiment was repeated with German cockroaches, greater toxic effects and more rapid effects were observed. It appears that some degradation occurs in toxic effects with time (Table 4).

                  TABLE 4                                                          ______________________________________                                         Effect of 3-B (1 mg/jar) when applied                                          to surfaces on American cockroaches                                            exposed to these surfaces at                                                   different times after surface                                                  treatment.                                                                     Time elapsed after                                                                            Time required to kill                                           3-B application                                                                               100% of insects                                                 (days)         (days)                                                          ______________________________________                                         1              1                                                               2              3                                                               3              6                                                               5              8                                                                7*            10 (6/10 died)                                                  ______________________________________                                          *Only 6 insects died when 10 cockroaches were exposed to pretreated jars       days after 3B application.                                               

The toxic effects shown in Table 4 could be protracted by combining oils with different characteristics. Eugenol, one of the ingredients of 3-B, was dissolved in galoxolide, a perfume oil that imparted longer lasting properties related to evaporation and oxidation to the pesticide properties of eugenol. Mixtures having 30%-60% galoxolide and 40%-70% eugenol by weight have been effective. As an example, eugenol and galoxolide were mixed in equal parts, 1 mg each, in 10 ml of acetone and agitated vigorously for two minutes and then applied (1 mg of mixture/jar) as above to small jars and all surfaces covered. The data of this example (Table 5) show that the lethal effect of the mixture was extended and enhanced.

                  TABLE 5                                                          ______________________________________                                         Time elapsed after Time required to                                            eugenol/galoxolide* application                                                                   kill 100% of insects                                        (weeks)            (days)                                                      ______________________________________                                         1                  1                                                           2                  1                                                           3                  1                                                           4                  1                                                           ______________________________________                                          *1, 3, 4, 6, 7, 8hexahydro-4, 6, 6, 7, 8,                                      8hexamethyl-cyclopenta-gamma-2-benzopyran                                

Cockroach abdominal nerve cord showed the second highest uptake of octopamine of all tissues studied [J. Neurochem 54 479-489 (1990)]. The high efficacy of the chemicals of the present invention in the "walk across" study is attributed to the high concentration of octopamine receptors on the ventral nerve cord in close proximity to the hind legs.

Since the toxic signs produced by 3B did not indicate cholinergic action patterns, other major possibilities such as GABA receptor, octopamine receptor/biogenic amine binding, [Ca² +], or mitochondrial respiratory poison were considered:

1. GABA receptor-chloride channel study: This site of action is known to be one of the major action sites for a number of insecticides. When the action of 3B was tested, 3H-EBOB ([³ H]n-propyl bicyclo ortho benzoate) and 35S-TBPS (bicyclo phosphorous esters) were used. The former ligand was used based on the findings that EBOB has been shown to be both a highly toxic and high affinity radioligand for the GABA-receptor convulsant binding site in insects. It also shows identical or overlapping binding sites with seven classes of insecticides: trioxabicyclooctanes, dithianes, silatranes, lindane, toxaphen, cyclodienes and picrotoxinin. The TBPS has been hindered by poor toxicological relevance and binding affinity in insects. Both radioligands were used in this study for comparison. As shown in Table 6, 3B induced no antagonistic action on the binding affinity of 3H-EBOB or 35S-TBPS at concentrations of 3B ranging from 10 μM to 10 NM. Only at 100 μM was a significant effect seen. However, at such a high concentration (100 μM), the specificity for the site of action is unlikely. In contrast, heptachlorepoxide alone and a mixture of Endosulfan I (60%) and Endosulfan II (40%) as a positive standard were highly active even at 10 Nm. These data indicate that this assay system works poorly with 3B, and that the lack of its action is due to its inaccessibility to the GABA receptor site. The fact that the cyclodiene resistant London strain showed cross-resistance to 3B supports the above data and rules out GABA receptor as the target or site of action for 3B.

                  TABLE 6                                                          ______________________________________                                         Effect of 3B on GABA-receptor binding                                          Tested      dpm per 200 μg synaptosomal membrane protein                    Concentration                                                                              (X ± SD)                                                        μM       .sup.3 H-EBOB .sup.35 S-TBPS                                       ______________________________________                                         3-B (μM)                                                                    0            2558 ± 159                                                                               3361 ± 297                                        0.01        2069 ± 98  2991 ± 101                                        0.10         2069 ± 198                                                                               2939 ± 111                                        1.0         2088 ± 76  2917 ± 85                                         10           2111 ± 151                                                                               3001 ± 173                                        100         2109 ± 88  2985 ± 203                                        Endosulfan Mix μM                                                           0            2558 ± 159                                                                               3361 ± 297                                        0.01        1886 ± 71  2477 ± 162                                        0.1         1009 ± 83  1358 ± 101                                        1.0          350 ± 35  552 ± 43                                          10           255 ± 29  360 ± 15                                          100          241 ± 18  285 ± 11                                          Hepatachlorepoxide                                                             μM                                                                          0.001       2010 ± 91  2470 ± 188                                        0.01        1583 ± 77  2000 ± 75                                         0.10        1221 ± 63  1699 ± 109                                        1.0         1142 ± 85  1493 ± 99                                         10           591 ± 41  685 ± 66                                          Unlabeled Ligand μM                                                         0.001        499 ± 34  533 ± 25                                          0.01         346 ± 29  381 ± 17                                          0.10         206 ± 15  211 ± 19                                          ______________________________________                                          Control value (solvent alone) was 2558 ± 159 × 3361 ± 297 for      EBOB × TBPS, respectively.                                         

2. Octopamine receptor/biogenic amine binding site: Biogenic amines are known to carry out a number of physiological functions through their specific receptors in insects. The octopamine receptor is the most dominant biogenic amine receptor in insects. Certain acaricides, such as chlordimeform, are known to act on octopamine receptors, causing a variety of symptoms, including behavioral changes. When 3B was incubated directly with a homogenate of the nerve cord of American cockroaches, a significant increase in cylical AMP (Camp) was found at a dosage of 1 μM. The chemical octopamine was used as a positive control and induced a significant increase in Camp at 1 μM. Additional evidence that octopamine is the main site of action of 3B is that in co-treatment of 3B and octopamine, 3B abolished the octopamine-induced increase in cAMP.

To confirm that 3B is an octopamine receptor toxicant, two important biomarkers were measured: heart beats/30 seconds and cAMP--dependent protein kinase (PKA) activity. These two are considered particularly important in identifying octopamine receptor activity as the cockroach heart has been shown to have a high concentration of octopamine receptors. When a 200 μg/insect concentration of 3B was applied to the sternum region of the alive and intact American cockroach, a significant increase was seen in heart beats/30 seconds and this was accompanied with an increase in cAMP. As before, higher concentrations of 3B resulted in a decrease in heart beats (Table 7).

                  TABLE 7                                                          ______________________________________                                         Effect of 3-B on American cockroach                                            heart beats/30 seconds.                                                        Tested Doses, μg                                                                         Before Treatment                                                                           30 Min. After Treatment                               ______________________________________                                         control      55 ± 1.9 55 ± 3.2                                           200          55 ± 2.1 71 ± 4.5                                           300           58 ± 0.81                                                                               67 ± 1.63                                         600           57 ± 0.47                                                                              41 ± 2.4                                           900          53 ± 1.9 38 ± 2.8                                           octopamine (20 μg)                                                                       54 ± 2.1 75 ± 3.6                                           chlordimeform (20 μg)                                                                    51 ± 1.4 69 ± 2.5                                           (positive control)                                                             ______________________________________                                    

In addition, when 3B was incubated with synaptasomal preparation from American cockroach heads, a significant increase in PKA activity was found, which is consistent with the above conclusion (Table 7).

                  TABLE 8                                                          ______________________________________                                         In Vivo effect to 3-B (200 μg/roach)                                        on the activity of PKA in                                                      synaptosomal membrane of American                                              cockroaches.                                                                             dpm/1 nmol of Kemptide/5 min.                                                  X ± SD                                                            ______________________________________                                         Control   1753 ± 57                                                         3-B       4008 ± 201                                                        ______________________________________                                    

3. Possible role of [Ca²⁺ ]: Because of the locomotive difficulties noticed among cockroaches treated with 3B at the ventral sternum region, it was postulated that increases in intramuscular levels of Ca²⁺ could be involved in the contractions of hind legs. For this purpose, mammalian cell line PC12 rat chromaffin adrenal cells was used. This cell line is known to mimic neuronal cells, particularly catecholaminergic neurons and has been used as a model for Ca2+ induced presynaptic transmitter release phenomena. As shown in FIG. 1 when 3B was added at 100 μM to these cells (at A), there was no change in intracellular free Ca²⁺. [Ca²⁺ ] concentration inside PC12 cells was found using spectrofluorometric measurements with Fura 2/AM (a cell penetrating fluorescence probe for free Ca²⁺). A standard positive control, thapsigargin clearly increased the [Ca²⁺ ]i (at B) even at 500 Nm. Also, 10 μM ionomycin, a Ca²⁺ -ionophore (at C) produced the expected increase of Ca²⁺ entry. These results showed that 3B shows no ability to regulate any type of calcium homeostasis in mammalian cells.

The effect of the chemicals of the present invention on the rat brain tissue cells was as follows where cyclical AMP generation is measured as dpm/mg protein

    ______________________________________                                         control         5395 ± 43                                                   3-B             5411 ± 391                                                  terpineol       5399 ± 219                                                  eugenol         5461 ± 488                                                  phenyl ethyl alcohol                                                                           5499 ± 415                                                  ______________________________________                                    

Thus, no change was produced in the neurotransmission system of a mammal. These data confirm the lack of neurotoxicity of these essential oils in mammals.

4. Mitochondrial/respiratory poison: Another possible mode of action is that of mitochondrial or respiratory poisoning. It has been observed that all mitochondrial poisons induce hyperactivity at some point in their action. However, when American cockroaches were treated with a topical application of 3B, no hyperactivity or hyper excitation was observed at any stage of poisoning at all concentrations used. On the other hand, when American cockroaches were exposed to pre-coated jars with 1 mg of a 60% solution of 3B, the insects showed hyper excitation. These data suggest that the mode of entry is a determining factor in the mode of action of these essential oils. These observations support the idea that 3B is not a type of poison that attacks the Na⁺ channel as a main target. The fact that the Kdr-resistant strain (with a mutated Na⁺ channel making it insensitive to DDT and pyrethroids) did not show cross-resistance to 3B also supports this diagnosis.

Under the above circumstances, where all of the known major sites of action for insecticides were found to be insensitive to 3B, except the antagonistic effect on the octopamine receptor, the possibility was considered that the mode of action of this group of chemicals is totally novel.

It was noted that the toxicity of 3B varies according to the site of application, at least in the case of the American cockroach. Such an observation indicates that 3B is likely to be not so systemic in action, and if one site produces a high toxicity, its target is likely to be located very close to that site of application. In view of the locomotive difficulty observed in American cockroaches treated at the central sternum, it was reasoned that the thoracic ganglia could be affected. When 3B (250 mg. in 0.4 μl acetone) was injected into the thorax of an alive cockroach and its hind leg contractions were induced by an external electrical stimulus applied to the outside of the body, the ability of the leg muscles to respond to stimuli totally disappeared within one minute (FIG. 2). When 3-B was applied to the leg in isolation by directly giving electrical stimuli, no effect of 3B was found (FIG. 3). These data suggest that the effect of 3B is on the nervous system and not on the muscles. Control tests using acetone (0.4 μl) without any 3-B showed no effects (FIGS. 4 and 5). This possibility was further tested using the 6th abdominal ganglion and studying transmission of signals from the mechanoreceptors of the cerci generated by air puffing. For this purpose, the abdominal cavity was opened, exposing the entire abdominal nerve cord. A suspension solution of 3B in 200 μl insect saline was directly applied to the entire abdominal nerve cord. The results were drastic, producing complete blockage of transmission at 250-500 PPM within 5 minutes from the time of application (FIG. 6). Even at 10 PPM the blocking effect of 3B was apparent, producing visible effects in 15 minutes. These results clearly indicate that 3B is a nonsystemic nerve blocker.

A further study was conducted on the cockroach to determine the effect of other chemicals of the present invention on the heart rate of the insects. The experiment conditions were as previously described in applying 300 μg of the respective chemical to the entire abdominal nerve cord. Each insect group was comprised of three individuals. All heart rates were observed and counts were recorded three times for each individual, i.e., nine counts before introduction of the chemical, and nine counts 30 minutes after application of approximately 300 μg of chemical. The data from these heart beat tests agreed with the previously described "walk-across" test. The following chemicals showed measurable change in heart rate thirty (30) minutes after application of the chemical: terpineol, eugenol, phenyl ethyl alcohol, benzyl acetate and benzyl alcohol.

Also the following mixtures showed measurable change in heart rate: terpineol with eugenol, terpineol with phenyl ethyl alcohol and eugenol with phenyl ethyl alcohol.

The toxicity of the individual chemicals was also determined by topical application to early 4^(th) instar Asian armyworms (Spodoptera litura) (15-20 mg live wt) and measurement after 24 hours (Table 9).

                  TABLE 9                                                          ______________________________________                                         Toxicity of chemicals of the present                                           invention.                                                                                        95% con-         95% con-                                            LD50      fidence   LD90   fidence                                    Compound (μg/larva)                                                                            interval  (μg/larva)                                                                         interval                                   ______________________________________                                         α-terpineol                                                                       156.0     148.7-163.7                                                                              206.4  190.4-249.9                                eugenol  157.7     149.9-165.8                                                                              213.0  194.8-263.3                                cinnamic LD50 > 250                                                            alcohol                                                                        (+)-terpinen-                                                                           130.4     121.8-139.5                                                                              205.8  180.2-283.8                                4-ol                                                                           (-)-terpinen-                                                                           122.9     108.1-139.7                                                                              276.0  202.4-583.9                                4-ol                                                                           carvacrol                                                                               42.7      37.7-48.3  73.8  55.7-142.0                                 D-pulegone                                                                              51.6      49.0-54.4  69.7  62.3-91.3                                  t-anethole                                                                              65.5      61.7-69.6  98.8  88.4-129.1                                 thymol   25.5      22.9-28.3  46.8  38.5-74.5                                  citronellal                                                                             111.3     103.9-119.1                                                                              153.4  130.8-223.5                                ______________________________________                                    

In establishing the LD50 dose, it was noted that all larvae treated with pulegone, even at the lowest doses tested, were almost immediately paralyzed. However, at lower doses, many or most of these larvae overcame this effect, whereas at higher doses larvae succumbed. To determine if the observed sublethal toxicity resulted in any long-term effect, subsequent growth of larvae treated at the three lowest doses (20, 31 and 49 μg per larvae) was monitored and compared this to growth of control (untreated larvae) at 72 and 100 hours after treatment (Table 10).

                  TABLE 10                                                         ______________________________________                                         Growth of larvae exposed to chemicals                                          of the present invention.                                                                 Control 20 μg/larva                                                                           31 μg/larva                                                                         49 μg/larva                            ______________________________________                                         Mortality (5)                                                                             0       0         2       52                                        Live wt. - 72 hr.                                                                         148 mg. 109       81      52                                        Live wt. - 110 hr.                                                                        363 mg. 289       248     163                                       ______________________________________                                    

The results indicate that subsequent larval growth of survivors of the highest dose is dramatically retarded. More importantly, the effect is also seen (and significantly so) at the two lowest doses, which themselves produced almost no mortality. Therefore, exposure to even sublethal doses can have significant consequences for larvae.

Dry powder formulations were prepared using the chemicals of the present invention. The examples listed below are for the 3B mixture as previously described (cinnamic alcohol, eugenol and alpha terpineol). However, these examples are for illustrative purposes only and do not limit the range of active chemicals. Other mixtures and individual chemicals may also be used. Suggested mixtures are phenyl ethyl alcohol, benzyl alcohol, eugenol and alpha terpineol (3C) and benzyl acetate, benzyl alcohol, phenyl ethyl alcohol, cinnamic alcohol and alpha terpineol (3D). The mixtures listed herein are not limiting but are typical and the present invention is not limited to these mixtures.

The procedure was to place the powder components into a 500 ml dish and apply the chemical (usually a liquid) on the powder. The powder and chemical mixed in the container were placed on an electric tumbler to dry for approximately 30 minutes. Approximately 1 cc of the resulting dry powder was applied to a Whatman No. 1 filter paper in a 9 cm Petri dish. The dust was spread evenly with a camel hair brush. A control was used which consisted only of the powder components without the chemical of the present invention.

The following compositions were prepared: (Tables 12a-12c)

                  TABLE 12a                                                        ______________________________________                                         % by weight                                                                               amount        components                                            ______________________________________                                         40         20 gr         diatomaceous earth                                    20         10 gr         calcium carbonate                                     20         10 gr         sodium bicarbonate                                    10          5 gr         Hi-Sil 233                                            10          5 gr         active ingredient*                                    ______________________________________                                    

                  TABLE 12b                                                        ______________________________________                                         % by weight   amount         components                                        ______________________________________                                         40            20 gr          diatomaceous earth                                23            11.5 gr        calcium carbonate                                 20            10 gr          sodium bicarbonate                                10            5 gr           Hi-Sil 233                                        7             3.5 gr         active ingredient*                                ______________________________________                                    

                  TABLE 12c                                                        ______________________________________                                         % by weight   amount         components                                        ______________________________________                                         40            20 gr          diatomaceous earth                                25            12.5 gr        calcium carbonate                                 20            10 gr          sodium bicarbonate                                10            5 gr           Hi-Sil 233                                         5            2.5 gr         active ingredient*                                ______________________________________                                          *active ingredient was a mixture of the chemicals of the present               invention.                                                               

The mixtures were tested by placing ten (10) common fire ants within respective Petri dishes which were then covered. The time for irreversible knockdown (KD) to occur (KT) was determined from periodic irregular observations. The insects were considered KD when they were on their back, or could be turned onto their back and could not right themselves within at least two (2) minutes. KT-50 and KT-90 (time for 50% and 90% KD respectively) were calculated by interpolation of KD between times when data was collected. The 10% mixture (Table 12a) had a KT 50% of 5 min. 50 sec. and a KT 90% of 6 min 40 sec. For the 7% mixture (Table 12b) KT 50% was 3 min. 40 sec. and KT 90% was 4 min. 40 sec. The 5% mixture (Table 12c) had KT 50% of 2 min. 33 sec. and KT 90% of 3 min. 45 sec.

The above data are examples which clearly demonstrate the effectiveness of the mixture of chemicals over concentrations ranging from 5%-10% by weight of the active ingredients. These are typical examples, but are not limiting. Concentrations as low as 0.1% by weight have also been shown to be effective for some individual chemicals and for various mixtures of chemicals. Also much higher concentrations can be used.

As an example of a high concentration, a emulsifiable concentrate has the following formulation:

    ______________________________________                                         Ingredient          Purpose     wt. %                                          ______________________________________                                         3B                  active ingredient                                                                          90.0                                           Sodium dodecyl benzene sulfonate                                                                   emulsifier  6.6                                            Sodium C12-15 Pareth-3 sulfonate                                                                   emulsifier  2.0                                            POE 20 Sorbitan Monooleate                                                                         emulsifier  1.4                                            ______________________________________                                    

    ______________________________________                                         Ingredient       Purpose     wt. %                                             ______________________________________                                         3B               active ingredient                                                                          90.0                                              Caster oil (40 mol EO)                                                                          emulsifier  10.0                                              ______________________________________                                    

The concentrate is diluted with up to 50 to 70 parts of water to 1 part of concentrate to provide an effective aqueous medium. Although these examples are for the 3B and 3C mixtures, the formulation is not so limited and an emulsifiable concentrate may be prepared from any of the individual chemicals of the present invention or any combination of mixtures of the individual chemicals.

The emulsifiable concentrate 3B mixture was diluted in tap water and sprayed onto cabbage and bean plants to assess efficacy against Asian armyworms and two-spotted spider mites (Tetranychus urticae) respectively. All values are based on a minimum of four doses with five replicates and ten armyworms or thirty adult and/or deutonymph mites per replicate. Values reported represent the ratios of water to formulation (i.e., dilution rate)(e.g., LD50 of 46.8 means a ratio of water to emulsifiable concentrate of 46.8:1). Mortality was assessed at 24 hours (Table 13).

                  TABLE 13                                                         ______________________________________                                         Mortality of emulsifiable mixture of                                           3-B mixture                                                                    ______________________________________                                         3-day old Asian armyworm                                                                        LD50 (95% C.I.) = 46.8 (44.0-49.8)                                             LD90 (95% C.I.) = 30.6 (27.2-40.7)                            5-day old Asian armyworm                                                                        LD50 (95% C.I.) = 53.0 (49.6-56.7)                                             LD90 (95% C.I.) = 33.7 (29.7-45.6)                            two-spotted spider mite                                                                         LD50 (95% C.I.) = 82.3 (76.5-88.5)                                             LD90 (95% C.I.) = 55.9 (49.3-75.5)                            ______________________________________                                    

The diluted emulsifiable concentrate 3B formulation shows good efficacy against both ages of armyworms, and even better efficacy against spider mites. Five-day old armyworms are approximately the same size as mature (last instar) diamondback moth larvae, a potential target species. Also of importance, it was observed that sublethal concentrations clearly deter feeding and therefore plant damage. At a dilution of 50:1, 60:1 and 70:1, larval mortality is only 64%, 42% and 12% respectively. However, there is minimal feeding damage from surviving larvae. As shown in FIG. 7, discs of cabbage plant leaves with 3-B emulsifiable concentrate diluted 50:1, 60:1 and 70:1 have areas consumed (ac) after 24 hours of less than 5%-30% as compared to a control (without 3-B) of greater than 80% area consumed.

Another example of a high concentration is a wettable powder having the following formulation:

    ______________________________________                                         Ingredient           Purpose     wt. %                                         ______________________________________                                         3B                   active ingredient                                                                          50.0                                          Silica, hydrated, amorphous                                                                         absorbent   41.5                                          Sodium alkyl naphthalene sulfonate                                                                  dispersant  3.0                                           Sodium dioctyl sulfosuccinate                                                                       wetting agent                                                                              0.5                                           Sodium dodecyl benzene sulfonate                                                                    emulsifier  5.0                                           ______________________________________                                    

One part of this powder is mixed with up to 30-50 parts of water to provide an effective pesticide. This example is for the 3B mixture but is not limited and may be prepared with any of the individual chemicals of the present invention or any combination of mixtures of the individual chemicals.

A waterproof dust may be prepared using any of the individual chemicals or a mixture of any of the chemicals of the present invention. The following formulation is representative in which the mixture 3C is equal parts by weight of benzyl alcohol, phenyl ethyl alcohol and terpineol:

    ______________________________________                                         Ingredient      Purpose     wt. %                                              ______________________________________                                         3C              active ingredient                                                                          5.0                                                Diatomaceous earth                                                                             bulking agent                                                                              75.0                                               Hydrophobic silica                                                                             absorbent   20.0                                               ______________________________________                                    

The following formulations represent two ready to use sprays representative of mixtures 3B and 3C but may be used for other mixtures and for individual chemicals of the present invention:

    ______________________________________                                         Ingredient        Purpose     wt. %                                            ______________________________________                                         3B                active ingredient                                                                          5.0                                              Caster oil (40 mol EO)                                                                           emulsifier  2.7                                              POE 20 Sorbitan Monooleate                                                                       emulsifier  0.6                                              Isopropylamine alkyl benzene                                                                     emulsifier  1.7                                              sulfonate                                                                      Water             diluent     90.0                                             3C                active ingredient                                                                          5.0                                              Caster oil (40 mol EO)                                                                           emulsifier  2.7                                              POE 20 Sorbitan Moncoleate                                                                       emulsifier  0.6                                              Isopropylamine alkyl benzene                                                                     emulsifier  1.7                                              sulfonate                                                                      Water             diluent     90.0                                             ______________________________________                                    

The individual chemicals of the present invention and mixtures may also be used as an aerosol spray. While not limited thereto, the following formulation is typical:

    ______________________________________                                         Ingredient        Purpose     wt. %                                            ______________________________________                                         3C                active ingredient                                                                          5.0                                              Propanol          solubilizing agent                                                                         1.5                                              Carbon dioxide    propellant  3.5                                              Isoparaffinic hydrocarbon                                                                        solvent     90.0                                             ______________________________________                                    

A pesticidal shampoo has the following composition where the active ingredient is an individual chemical of the present invention or mixture of chemicals:

    ______________________________________                                         Ingredient         wt. %                                                       ______________________________________                                         Active ingredient  0.5-10.0                                                    Sequestrant for hard water                                                                        0.5-3.0                                                     Emulsifier(s)      1.0-5.0                                                     Thickener(s)       0.5-5.0                                                     Foam stabilizer    0.5-2.0                                                     Detergent          5.0-20.0                                                    Buffer             0.1-2.0 (to required pH)                                    Dye/colorant       0.01                                                        Preservative       0.01-1.0                                                    Deionized water    to 100%                                                     ______________________________________                                    

Gel formulations have been prepared as follows:

    ______________________________________                                         Blue Color                                                                     ______________________________________                                         3.00%                Active ingredient                                         15.00%               Ethanol                                                   0.42%                Carbopol 934                                              0.42%-10%            Sodium hydroxide in water                                 81.16%               Water                                                     ______________________________________                                         Yellow Color                                                                   ______________________________________                                         3.00%                Active ingredient                                         30.00%               Ethanol                                                   0.42%                Carbopol 934                                              0.42%-10%            Sodium hydroxide in water                                 66.16%               Water                                                     ______________________________________                                    

The active ingredient is an individual chemical of the present invention or a mixture thereof.

A dry powder formulation consists of mixing an alkaline earth metal carbonate, such as calcium carbonate, an alkali metal bicarbonate, such as sodium bicarbonate, the active ingredient, an absorbent material, such as diatomaceous earth and a bulk agent such as HiSil 233.

The relative concentrations of the mixture are preferably about 20%-30% alkaline earth metal carbonate, 15%-25% alkali metal bicarbonate, 0.1%-5% active ingredient, 30%-50% absorbent material and 5%-15% bulk agent (all by weight). The granules of powder preferably are ground to a size under 100 microns.

The active ingredients may be individual chemicals of the present invention or mixtures thereof.

In all of the above recited examples, it may be desirable to add a trace amount (less than 2%) of a material to provide a pleasant odor, not only for aesthetic reasons but also to identify the areas of a building which have been treated. The pleasant odor may be vanilla, cinnamon, floral or other odors which are acceptable to consumers. The pleasant odors are not limited to the examples given herein.

Obviously, many modifications may be made without departing from the basic spirit of the present invention. Accordingly, it will be appreciated by those skilled in the art that within the scope of the appended claims, the invention may be practiced other than has been specifically described herein. 

What is claimed is:
 1. A method for interacting with the neurotransmitters of octopamine receptor sites in invertebrates and/or larvae thereof, to thereby kill and/or affect the feeding habits and growth of the invertebrates and/or larvae thereof without similarly affecting mammals, fish or fowl, the method comprising:selecting an affector agent based on the ability of the affector agent to interact as an agonist or antagonist on the neurotransmitters of the octopamine receptor sites in the invertebrates and/or larvae thereof, the affector agent comprising a naturally occurring compound having a six member carbon ring structure, with the carbon ring being substituted by at least one oxygenated functional group and the structure of the affector agent is devoid of nitrogen; applying to the invertebrates, their larvae and/or their habitat, a pesticidal composition of a carrier with said affector agent, wherein the affector agent is present in the pesticidal composition in an effective amount to interact as an agonist or antagonist on the octopamine receptor sites to kill or affect the feeding and growth of the invertebrates and/or their larvae and is not harmful to mammals, fish or fowl.
 2. The method of claim 1, comprising applying the mixture to insects, army worms, arachnids, larvae thereof, and/or their habitat.
 3. The method of claim 1, wherein the affector agent is selected from the group consisting of anethole, benzyl acetate, benzyl alcohol, carvacrol, cinnamic alcohol, eugenol, phenyl ethyl alcohol, pulegone, alpha terpineol, and mixtures thereof.
 4. The method of claim 3, wherein the mixture is selected from a group consisting of cinnamic alcohol, eugenol with alpha terpineol; benzyl alcohol, phenyl ethyl alcohol, eugenol with alpha terpineol; and benzyl acetate, benzyl alcohol, phenyl ethyl alcohol, cinnamic alcohol with alpha terpineol.
 5. The method of claim 4, wherein the range of weight percent of the mixture is cinnamic alcohol 20%-35%, eugenol 10%-50% and alpha terpineol 10%-50%.
 6. The method of claim 1, further comprising adding to the mixture a material having a pleasant odor.
 7. The method of claim 1, wherein the carrier is water having at least one emulsifier therein.
 8. The method of claim 1, wherein the carrier is a wettable powder.
 9. The method of claim 1, wherein the carrier is a waterproof dust.
 10. The method of claim 1, wherein the carrier is an aerosol.
 11. The method of claim 1, wherein the carrier is a dry powder.
 12. A method for killing and/or affecting the feeding habits and growth of invertebrates, and/or larvae thereof, the method comprising:selecting affector agents based on the agonist or antagonist activity of the affector agents with neurotransmitters of octopamine receptor sites in the invertebrates; applying to the invertebrates, larvae thereof and/or their habitat the affector agents as a blend of cinnamic alcohol, eugenol and alpha terpineol, the blend being mixed with a carrier to produce a substantially uniform pesticidal composition wherein the blend of affector agents is present in the pesticidal composition in an effective amount to interact as an agonist or antagonist on the octopamine receptor sites to kill or affect the feeding habits and growth of the invertebrates and/or their larvae and is not harmful to mammals, fish or fowl.
 13. The method of claim 12, wherein the range of weight percent of the blend is cinnamic alcohol 20%-35%, eugenol 10%-50% and alpha terpineol 10%-50%.
 14. The method, according to claim 1, wherein the mixture comprises an oil.
 15. The method of claim 14, wherein the oil is galoxolide and the affector agent is eugenol.
 16. The method according to claim 15, wherein the mixture comprises approximately 30%-60% by weight of galoxolide and approximately 40%-70% by weight of eugenol.
 17. The method of claim 14, comprising approximately 1 mg of the mixture is over an area of approximately 10 sq. cm.
 18. A method for killing and/or affecting the feeding habits and growth of invertebrates and/or larvae thereof, the method comprising: selecting affector agents based on the selected affector agents interacting as an agonist or antagonist with neurotransmitters of octopamine receptor sites in the invertebrates;applying to the invertebrates, their larvae and/or their habitat, a pesticidal composition comprising a carrier and the affector agents as a blend selected from the group consisting of: (1) cinnamic alcohol, eugenol and alpha terpineol; (2) benzyl alcohol, phenyl ethyl alcohol, eugenol and alpha terpineol; (3) phenyl ethyl alcohol, eugenol and alpha terpineol; and (4) benzyl acetate, benzyl alcohol, phenyl ethyl alcohol, cinnamic alcohol and alpha terpineol; wherein the blend of affector agents is present in the pesticidal composition in an effective amount to interact as an agonist or antagonist on the octopamine receptor sites to kill or affect the feeding habits and growth of the invertebrates and/or their larvae and is not harmful to mammals, fish or fowl.
 19. The method of claim 3, wherein the affector agent is selected from the group consisting of anethole, benzyl acetate, benzyl alcohol, carvacrol, cinnamic alcohol, citronellal, phenyl ethyl alcohol, pulegone, and mixtures thereof.
 20. The method according to claim 1, wherein the mixture is in the form of an emulsion.
 21. The method according to claim 12, comprising applying the pesticidal composition to insects, army worms, arachnids, larvae thereof, and/or their habitat, wherein the affector agents externally contact the insects, army worms, arachnids and larvae thereof.
 22. The method according to claim 18, comprising applying the pesticidal composition to insects, army worms, arachnids, larvae thereof, and/or their habitat, wherein the affector agents externally contact the insects, army worms, arachnids and larvae thereof.
 23. The method of claim 1, wherein action as an agonist or antagonist on the receptor sites is produced by external contact of the invertebrates and/or larvae with the affector agent. 